PTPSP
Concentration of samples
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Protocol for concentration of samples, written by Rosa Schier

  • Measure the concentration A280nm on the nanodrop
  • Put in a centrifugal filter unit Amicon Ultra (from Millipore) (attention to take the right size in kD)
  • Centrifuge 10min (can be shorter or longer) at 3900rpm repeat this until you have the volume you want (pay attention to precipitation)
  • Pool everything back and rinse the filter with 200uL of flow through (FT)
  • Measure the concentration again A280nm

 

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