Welcome to the PTPSP Wiki

On this WIKI you will find information on common things we do in the lab such as, stocks, antibiotics, simple protocols.

You can use the Sitemap to look at all the pages or find them below. You can also search all page on the right.

You can easily add new pages by clicking 'New Page'. These pages will show up on the Sitemap until they are added here.

Common Lab Housekeeping

Antibiotics and Stock solutions

Common Purification buffers

Chemical competent Ecoli cells - Rubidium Chloride method

Chemical competent Ecoli cells - TSS method

M9 Medium for NMR

Bacmid LB-Agar plates

Molecular Biology

PCR (Polymerase Chain Reaction)

SDS-PAGE

Mammalian and Insect Cell Culture

Waking up / Defrosting mammalian cells

Freezing mammalian cells

Transfecting HEK293E Cells (PEI PTPSP method)

Transfecting ExpiCHO (PEI PTPSP method)

Growing Bacterial Cultures

Growing bacterial cultures for protein expression

Purification

Purification of antibodies and Fc-containing proteins (Eukaryotic) by batch method

Purification of His-tag containing secreted proteins (Eukaryotic) by batch method

Regeneration of Protein A beads

Purification of His-tag containing proteins (Ecoli)

Purification of Twin-Strep tag containing proteins

Site-specific Biotinylation via the Avitag

Dialysis in Buffer

Akta care and mainteance

Biophysical

SEC-MALS

BLI

SPR

CD

ITC

Stunner

Refeyn Mass Photometer

Thermofluor assay

Crystallography

Crystal Buffer Screens