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Regeneration of protein A beads
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Regeneration of protein A beads, written by Rosa Schier

  • Let the beads (Mabselect beads from Amersham BioScience) settle
  • Decant the storage 20% Ethanol
  • Wash 2x with NaOH 50mM and each time let it spin around 10min or if the volume is too big let settle
  • Wash 2x with NaCl 1M (spin or settle)
  • Wash 2x with H2O (spin or settle)
  • Optional: Wash in PBS
  • Store it in EtOH 20%
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