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2015/report/debugging/scattering
Light scattering is a physical process where light is forced to deviate from a straight trajectory. The bacteria's shape deviate the blue light and induces light scattering. We would like to know if the blue light, which excites GFP in bacteria at 475nm, is also detected by the sensor because it can’t distinguish between green and blue light. If it's the case the light detection would be higher and arsenic concentration would be distorted.
To determine if the sensor also detects blue light we would like to compare eGFP bacteria and lysed eGFP bacteria, since bacteria's shape is the first source of light scattering. To test this assumption we realized a cell lysis protocol.
Reference: http://en.wikipedia.org/wiki/Light_scattering