2015/report/debugging/test

We would like to determine if blue light is also present in green fluorescence detection. With this different test we want to quantify the blue light scattering. We analysed the light spectrum of the following samples to compare the amount of blue light and green fluorescence:

-Free GFP from cell lysis protocol

-eGFP bacteria

-Fluorescent dextran as negative control because we know that dextran does not scatter

-bacteria lysed without GFP (control)

We first tried to test this light scattering with the prototype I and finally we found and a better solution and built a new box.

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